Milky Spore Powder containes natural bacteria which infect white grubs (Japanese Beetle Larvae) in the soil of your lawn and garden. Only one application of the powder is required. Apply anytime of hte year the ground is not frozen. Compatible with most lawn chemicals, fertilizer, and grass seed. Apply 1 teaspoon every 4 feet in 4 foot rows before rain, or water in after application. Treats 2,500 square feet.